Suppression subtractive hybridization (or suppressive subtractive hybridization) is a technique that uses PCR to quickly compare the expression of mRNA from different samples, and show the relative difference in the concentration of these molecules.
See also
External links
- Overview at evrogen.com
- Munir S, Singh S, Kaur K, Kapur V. "Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells". Biol Proced Online 6: 94-104. PMID 15181476. link
- Diatchenko L, Lau Y, Campbell A, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov E, Siebert P (1996). "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries". Proc Natl Acad Sci U S A 93 (12): 6025-30. PMID 8650213.
- Galbraith E, Antonopoulos D, White B (2004). "Suppressive subtractive hybridization as a tool for identifying genetic diversity in an environmental metagenome: the rumen as a model". Environ Microbiol 6 (9): 928-37. PMID 15305918.
