A mother superior is an abbess or other nun in charge of a Christian religious order or congregation, a convent or house of women under vows. Mother superior may also refer to:
- Mother Superior (band), a rock band who became ¾ of Rollins Band circa 2000
- Mother Superior, a short film about meth-moms in Salt Lake City, Ut. which was in the 2007 Sundance Film Festival
- "Mother Superior", a song on the album Good Apollo, I'm Burning Star IV, Volume Two: No World for Tomorrow by the progressive rock band Coheed & Cambria
- Sin (Marvel Comics), Synthia Schmidt or Mother Superior, the daughter of the Red Skull in Marvel Comics
- "Mother Superior", the nickname of the heroin drug-dealer Johnny Swann in Irvine Welsh's novel Trainspotting
