Darkest Night may refer to: Music
- "Darkest Night", a song on the 1995 album The Biz by The Sea and Cake
- "Darkest Night", a song on the 2001 album Green Room Blues by Deluxtone Rockets
- "Darkest Night", a song on the 2003 album Nazarene Crying Towel by Lost Dogs
- "Darkest Night", a song on the 2005 album shadows are security by As I Lay Dying
- "Darkest Night", a heavily sampled song by French band Lafayette Afro Rock Band.
Television
- "Darkest Night", an episode of the fan film series Star Trek: Hidden Frontier (Episode 3, Season 5)
- "In Darkest Night", a second-season episode of the animated series Beast Machines
Literature
- Batman: In Darkest Knight, a 1994 Batman comic book
- Darkest Knight, a 1996 Star Wars novel by Kevin J. Anderson
