Cbl gene

Cas-Br-M (murine) ecotropic retroviral transforming sequence
PDB rendering based on 1b47.
Available structures: 1b47, 1fbv, 1yvh, 2cbl, 2oo9
Identifiers
Symbol(s)	CBL; C-CBL; CBL2; RNF55
External IDs	OMIM: 165360 MGI: 88279 Homologene: 3802
Gene Ontology Molecular Function: • transcription factor activity • ubiquitin-protein ligase activity • signal transducer activity • calcium ion binding • protein binding • zinc ion binding • ligase activity Cellular Component: • nucleus • plasma membrane Biological Process: • cell surface receptor linked signal transduction • epidermal growth factor receptor signaling pathway • protein ubiquitination • positive regulation of receptor mediated endocytosis
RNA expression pattern
More reference expression data
Orthologs
	Human	Mouse
Entrez	867	12402
Ensembl	ENSG00000110395	na
Uniprot	P22681	na
Refseq	NM_005188 (mRNA) NP_005179 (protein)	XM_001001857 (mRNA) XP_001001857 (protein)
Location	Chr 11: 118.58 - 118.68 Mb	na
Pubmed search	[1]	[2]

Cbl (named after Casitas B-lineage Lymphoma) is a mammalian gene encoding several proteins involved in cell signalling and protein ubiquitination. Mutations to this gene have been implication in a number of human cancers, particularly acute myeloid leukaemia.

Discovery

In 1989 a virally encoded portion of the chromosomal mouse Cbl gene was the first member of the Cbl family to be discovered ^[1] and was named v-Cbl to distinguish it from normal mouse c-Cbl. The virus used in the experiment was a retrovirus known as Cas-Br-M, and was found to have excised approximately a third of the original c-Cbl gene from mice it was injected into. Sequencing revealed that the portion carried by the retrovirus encoded a tyrosine kinase binding domain, and that this was the oncogenic form as retroviruses carrying full-length c-Cbl did not induce tumour formation. The resultant transformed retrovirus was found to consistently induce a type of pre-B lymphoma, known as Casitas B-lineage lymphoma, in infected mice.

Structure

Full length c-Cbl has been found to consist of several regions encoding for functionally distinct protein domains:

• N-terminal tyrosine kinase binding domain (TKB domain): determines the protein which it can bind to
• RING-finger" motif: recruits enzymes involved in ubiquitination
• Proline-rich region: the site of interaction between Cbl and cytosolic proteins involved in Cbl's adaptor functions
• C-terminal ubiquitin-associated domain (UBA domain): the site of ubiquitin binding

This domain structure and the tyrosine and serine-rich content of the protein product is typical of an "adaptor molecule" used in cell signalling pathways.^[2]

Homologues

Three mammalian homologues have been characterized, which all differ in their ability to function as adaptor proteins due to the differing lengths of their C-terminal UBA domains:

1. c-Cbl: ubiquitously expressed, 906 amino acids in length.
2. Cbl-b: ubiquitously expressed, 982 amino acids long in length.
3. Cbl-c: lacks the UBA domain and is therefore only 474 amino acids in length. It is primarily expressed in epithelial cells however its function is poorly understood.

Interestingly, both c-Cbl and Cbl-b have orthologues in D. melanogaster (D-Cbl) and C. elegans (Sli-1), hinting at a long evolutionary path for these proteins^[2].

Functions

Ubiquitin ligase

Ubiquitination is the process of chemically attaching ubiquitin monomers to a protein, thereby targeting it for degradation. As this is a multi-step process, several different enzymes are involved, the final one being a member of the E3 family of ligases. Cbl functions as an E3 ligase, and therefore is able to catalyse the formation of a covalent bond between ubiquitin and Cbl's protein substrate - typically a receptor tyrosine kinase. The RING-finger domain mediates this transfer, however like other E3 ligases of the RING type no intermediate covalent bond is formed between ubiquitin and the RING-finger domain. The stepwise attachment of ubiquitin to the substrate receptor tyrosine kinase can lead to its removal from the plasma membrane and subsequent trafficking to the lysosome for degradation.

References

Further reading