| 3,3'-Diaminobenzidine | |
|---|---|
 |
|
| IUPAC name | 3,3',4,4'-Tetraamino- diphenyl |
| Identifiers | |
| CAS number | |
| RTECS number | DV8750000 |
| Properties | |
| Molecular formula | C12H14N4 |
| Molar mass | 214.27 g/mol |
| Melting point |
175–177 °C |
| Boiling point |
°C |
| Hazards | |
| MSDS | External MSDS |
| EU classification | Harmful (Xn) |
| NFPA 704 |
 1
2
2
|
| S-phrases | S36 |
| R/S statement | R20 R21 R22 R40 |
| LD50 | mouse, oral Acute: 1834 mg/kg. |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
|
3,3'-Diaminobenzidine (DAB) is a chemical compound. It is usually used as the water-soluble tetrahydrochloride in permanent immunohistochemical staining of nucleic acids and proteins.
Preparation
Diaminobenzidine is commercially available. It may be prepared by reacting 3,3'-dichlorobenzidine with ammonia with a copper catalyst at high temperature and pressure, followed by acidic workup to remove residual catalyst and starting material.[1]
Applications
Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark brown color. This is used to detect fingerprints in blood.[2]
References
- ^ US patent 3943175, "Synthesis of pure 3,3'-diaminobenzidine", granted 1976-03-09, assigned to Celanese Corporation
- ^ D.A.B. (Diaminobenzidine). Chesapeake Bay Division, International Association for Identification. Retrieved on 2007-11-09.
