Dictionary of Biological Psychology
An anatomical technique to localize radiolabelled markers in tissue sections (see RADIOLABEL). Radioisotopes may include 3H, 32P, 35S or 125I.
These markers are visualized either by laying X-ray film over histologically processed sections on slides (see HISTOLOGY), or by coating such slides with a photographic emulsion which provides better cellular resolution. Following exposure, the film or slides are developed as in photography, visualizing the labels as dark silver grains. For RECEPTOR BINDING autoradiography, a radioactive label is tagged to a LIGAND of interest. For IN SITU HYBRIDIZATION, complementary DNA, RNA or oligonucleotide probes are tagged with a radioisotope.
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