Polymerase Chain Reaction (Pcr) - Research Article from World of Microbiology and Immunology

This encyclopedia article consists of approximately 2 pages of information about Polymerase Chain Reaction (Pcr).

Polymerase Chain Reaction (Pcr) - Research Article from World of Microbiology and Immunology

This encyclopedia article consists of approximately 2 pages of information about Polymerase Chain Reaction (Pcr).
This section contains 532 words
(approx. 2 pages at 300 words per page)
Buy the Polymerase Chain Reaction (Pcr) Encyclopedia Article

PCR (polymerase chain reaction) is a technique in which cycles of denaturation, annealing with primer, and extension with DNA polymerase, are used to amplify the number of copies of a target DNA sequence by more than 106 times in a few hours. American molecular biologist Kary Mullis developed the idea of PCR in the 1970s. For his ingenious invention, he was awarded the 1993 Nobel Prize in physiology or medicine.

The extraction of DNA polymerase from thermophilic bacteria allowed for major advances in PCR technology.

PCR amplification of DNA is like any DNA replication by DNA polymerase in vivo (in living cells). The difference is that PCR produces DNA in a test tube. For a PCR to happen, four components are necessary: template, primer, deoxyribonecleotides (adenine, thymine, cytosine, guanine), and DNA polymerase. In addition, part of the sequence of the targeted DNA has to...

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This section contains 532 words
(approx. 2 pages at 300 words per page)
Buy the Polymerase Chain Reaction (Pcr) Encyclopedia Article
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