Dna Libraries - Research Article from Macmillan Science Library: Genetics

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The Importance of Vectors

Several types of DNA libraries have been developed for specific purposes, but all share some common features. The DNA fragments that make up the library are attached to other DNA sequences that are used as "handles" to maintain the fragments. These "handles," called vectors, allow the DNA to be replicated and stored, typically within model organisms such as yeast or bacteria.

Different types of vectors can be used to store DNA fragments of different lengths. For example, plasmid vectors can store small fragments (from a few hundred bases up to ten or twenty thousand bases of sequence), while viral vectors, or viral-plasmid hybrids such as cosmids, can store up to fifty thousand bases, and yeast artificial chromosome (YAC) vectors can store hundreds of thousands of bases. In general, plasmid-based vectors are the easiest to manipulate but store the smallest fragments. They are commonly used...

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This section contains 1,075 words
(approx. 4 pages at 300 words per page)
Buy the Dna Libraries Encyclopedia Article
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Macmillan Science Library: Genetics
Dna Libraries from Macmillan Science Library: Genetics. Copyright © 2001-2006 by Macmillan Reference USA, an imprint of the Gale Group. All rights reserved.
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