Clinical Chemistry, June 1st, 2007
General DNA analysis includes detection of targets, as well as identification and quantification of specific variants. Genomic DNA exists in the form of hotnoduplexes, with all corresponding base pairs being complementary, A:T and C:G. We call a double-stranded DNA (dsDNA) molecule a heteroduplex when it contains any noncomplementary base pairs. Conformational and thermodynamic changes produced by mismatched bases facilitate the detection of heteroduplexes. Denaturation of samples followed by hybridization to promote heteroduplex formation has been used to screen diploid DNA for heterozygous v...
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