The Scientist, January 13th, 2003
LAB CONSUMER
New products simplify alkaline lysis
Nucleic acid purification procedures have enjoyed a long history, beginning with the first isolation of "nuclein" in 1869 by Freidrich Meischer. In 1958, Matthew Meselson and Frank Stahl demonstrated sem icon servative replication of DNA using density-gradient centrifugation, an isolation method that became the cornerstone of recombinant DNA technology in the '70s and '80s. The technology was effective-producing high-- quality DNA-but it was not efficient.
The classical method of isolating plasmid DNA from bacterial cells, called the alkalin...
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