Plant Physiology, February 1st, 2004
Protein storage vacuoles (PSVs) are specialized vacuoles devoted to the accumulation of large amounts of protein in the storage tissues of plants. In this study, we investigated the presence of the storage vacuole and protein trafficking to the compartment in cells of tobacco (Nicotiana tabacum), common bean (Phaseolus vulgaris), and Arabidopsis leaf tissue. When we expressed phaseolin, the major storage protein of common bean, or an epitope-tagged version of [alpha ]-tonoplast intrinsic protein ([alpha ]-TIP, a tonoplast aquaporin of PSV), in protoplasts derived from leaf tissues, these prote...
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