Plant Physiology, March 1st, 2004
Vacuoles perform multiple functions in plants, and VCL1 (VACUOLESS1) is essential for biogenesis with loss of expression in the vcl1 mutant leading to lethality. Vacuole biogenesis plays a prominent role in gametophytes, yet is poorly understood. Given the importance of VCL1, we asked if it contributes to vacuole biogenesis during pollen germination. To address this question, it was essential to first understand the dynamics of vacuoles. A tonoplast marker, [delta ]-TIP[four dots above ]GFP, under a pollen-specific promoter permitted the examination of vacuole morphology in germinating pollen ...
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