Biophysical Journal, December 15th, 2006
ABSTRACT
Electron paramagnetic resonance (EPR) oximetry is being widely used to measure the oxygen consumption of cells, mitochondria, and submitochondrial particles. However, further improvement of this technique, in terms of data analysis, is required to use it as a quantitative tool. Here, we present a new approach for quantitative analysis of cellular respiration using EPR oximetry. The course of oxygen consumption by cells in suspension has been observed to have three distinct zones: pO^sub 2^-independent respiration at higher pO^sub 2^ ranges, pO^sub 2^-dependent respiration at low pO^s...
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