Biology Essay | Molecular Biology Experiment

This student essay consists of approximately 3 pages of analysis of Molecular Biology Experiment.
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Molecular Biology Experiment

Summary: Details an experiment that tests bacterial transformation. Provides background information on the situation, materials, and step-by-step method. Concludes that the results did not exactly match the hypothesis; the LB/Amp + solution had no cell growth.
Bacterial Transformation--Ampicillin Resistance

Problem/situation:

E. coli, or Escherichia coli, is a type of bacteria commonly used by molecular geneticists in recombinant DNA research. One reason is because E. coli has around 5 million DNA base pairs. The E. coli bacteria can be grown in Luria broth mixed with agar, otherwise known as LB agar. Some plasmids, such as the R plasmids, have genes for resisting antibiotics. Genes are transferred in bacteria by conjugation, a process similar to mating, transduction, a vector carrying info as a liason between 2 bacteria, and transformation. Transformation is the genetic information transfer into a cell by the direct uptake of DNA. Bacteria can take in DNA at a logarithmic rate, during which the cells are called competent. When the cells become competent, they can accept DNA introduced to another source.

In this lab, we are inserting plasmids, circular DNA molecules, that are resistant...

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This section contains 710 words
(approx. 3 pages at 300 words per page)
Buy the Student Essay on Molecular Biology Experiment
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