Summary:
Details an experiment that tests bacterial transformation. Provides background information on the situation, materials, and step-by-step method. Concludes that the results did not exactly match the hypothesis; the LB/Amp + solution had no cell growth.
Bacterial Transformation--Ampicillin Resistance
Problem/situation:
E. coli, or Escherichia coli, is a type of bacteria commonly used by molecular geneticists in recombinant DNA research. One reason is because E. coli has around 5 million DNA base pairs. The E. coli bacteria can be grown in Luria broth mixed with agar, otherwise known as LB agar. Some plasmids, such as the R plasmids, have genes for resisting antibiotics. Genes are transferred in bacteria by conjugation, a process similar to mating, transduction, a vector carrying info as a liason between 2 bacteria, and transformation. Transformation is the genetic information transfer into a cell by the direct uptake of DNA. Bacteria can take in DNA at a logarithmic rate, during which the cells are called competent. When the cells become competent, they can accept DNA introduced to another source.
In this lab, we.....
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