Three Sisters (play), a play by Anton Chekhov.

The Three Sisters (Aleutian Islands), Alaska

Three Sisters (Pittsburgh), a collection of three nearly identical bridges in Pittsburgh, Pennsylvania, United States.

An alternate name for the titular characters of Dario Argento's The Three Mothers, a trilogy of horror films.

Three Sisters (Oregon), a cluster of volcanoes in Oregon, USA.

Three Sisters (Glen Coe), a trio of ridges which are part of Bidean nam Bian in Glen Coe, Scotland.

Three Sisters (Alberta), a trio of peaks near Canmore, Alberta, Canada.

Three Sisters (film), a 1970 motion picture adaptation of the Chekhov play, directed by Laurence Olivier.

Three Sisters (Australia), a large rock formation in the Blue Mountains near Sydney, New South Wales, Australia.

Three Sisters (District of Columbia), three islands in the Potomac River in Washington, D.C., United States.

Three Sisters (agriculture), the three basic agricultural crops of indigenous peoples in North America.

The Three Sisters (Ireland), a group of three rivers in Ireland.

Three Sisters (British Columbia) a single mountain with three peaks near Fernie, British Columbia, Canada

Three Sisters Springs (Florida), on the Crystal River.

Three Sisters Recreation Area, a reclaimed conservation area and motorsport centre near Wigan, England.

Three Sisters (tomato), a variety of tomato.

The Three Sisters (Queensland), three islands in the Torres Strait North Queensland, Australia.

Tri Sestry, a volcano in the Kuril Islands.

Three Sisters (Georgia), a trio of peaks near Dahlonega in Lumpkin County, Georgia.

Drei Schwestern (German for 'Three Sisters'), a peak in Liechtenstein.