New Brunswick Southern Railway, part of the Canadian Pacific Railway

Washington Southern Railway (Washington) in Washington state

Southern Railway (Texas), part of the Atchison, Topeka and Santa Fe Railway

Utah Southern Railroad, part of the Union Pacific Railroad

West Virginia Southern Railway, part of the Norfolk and Western Railway

California Southern Railroad (possibly several companies of that name)

Minnesota Southern Railway, part of the Chicago, Milwaukee, St. Paul and Pacific Railroad

Quebec Southern Railway, part of the Delaware and Hudson Railroad

Virginia and Carolina Southern Railroad, part of the Atlantic Coast Line Railroad

Iowa Southern Railway, part of the Chicago and North Western Railway

Tennessee Southern Railroad, part of the Illinois Central Railroad

Iowa Southern Railroad, part of the Wabash Railway

New Jersey Southern Railroad and New Jersey Southern Railway, part of the Central Railroad of New Jersey

Tennessee and Carolina Southern Railway, part of the Southern Railway

Nebraska Southern Railway, part of the Missouri Pacific Railroad

Indiana Southern Railroad (never built)

Ohio Southern Railroad, part of the Detroit, Toledo and Ironton Railroad

Virginia Southern Railroad

Southern Railway of British Columbia

Austrian Southern Railway

Montana Southern Railway

Southern Railway (India)

The following railways or railroads are or were called the Southern Railway or Southern Railroad:

Southern (train operating company), a National Rail franchise operator in the United Kingdom

Southern Railway (Great Britain) in southern England (1923-1948)

Southern Railway (U.S.), now part of Norfolk Southern

Canada Southern Railway, part of the New York Central Railroad

Southern Railway and Navigation Company, a line of steamboats on the Ohio and Tennessee Rivers

Arkansas Southern Railroad, part of the Chicago, Rock Island and Pacific Railway

Georgia Southern Railroad, part of the Southern Railway

Kansas Southern Railway, part of the Atchison, Topeka and Santa Fe Railway

Southern Railroad of Long Island, part of the Long Island Rail Road

Southern Railroad (Michigan), part of the New York Central Railroad

Southern Railway and Light Company and Southern Railway and Traction Company, a street railway in Natchez, Mississippi

Southern Railway (St. Louis), a street railway in St. Louis, Missouri

Southern Railroad of New Jersey, a short line

Vermont Southern Railroad, part of the Boston and Maine Railroad

Washington Southern Railway (Virginia) in Virginia, south from Washington, D.C.