Middlesex (novel), by Jeffrey Eugenides that won the 2003 Pulitzer Prize for Fiction

"Middlesex" (poem), by John Betjeman

Middlesex School is a boarding school in Concord, Massachusetts

Middlesex County Cricket Club one of 18 first class cricket teams in the English County Championship

Middlesex University, London England

Middlesex, New Jersey, a borough

Middlesex, Vermont, a town

Middlesex, New York, a town

Middlesex, North Carolina

Middlesex Canal is in eastern Massachusetts

Middlesex, Jamaica is one of the three sub-divisions of Jamaica

Middlesex Township, Pennsylvania

Middlesex County, the name of six counties in North America

Middlesex is a traditional county of England, now absorbed by Greater London. Middlesex may also refer to: