Let There Be Light, a film by John Huston

"Let There Be Light", a fifth season episode of the fantasy television series Hercules: The Legendary Journeys

Let there be light, a Rugrats episode

Let there be light, the English translation of a line from the Bible

"Let There Be More Light", a song by Pink Floyd

Let There Be Light (short story), written by Robert A. Heinlein

Let There Be Light, a short story written by Arthur C. Clarke from the collection Tales of Ten Worlds

"Let There be Light", a song by 21st Century Schizoid Band on their album Live in Italy

Let There Be Light, the diary of Tony Gillan

"Let there be light", the first single from Mike Oldfield's album The Songs of Distant Earth