Publius Terentius Afer (d. 159 BC), Roman comic playwright better known as Terence

Afer ventus, another name for Lips, the Roman deity of the southwest wind. See Anemoi.

Orycteropus afer, aardvark

Antonius Guilelmus Amo Afer (1703–c.1759), alternate name for Anton Wilhelm Amo, Ghanaian-German academic

A song on the 1991 Enya album Shepherd Moons

Gaius Marius Victorinus Afer (4th cent.), also Gaius Marius Victorinus, Roman grammarian and rhetorician

Arnobius Afer (d. 4th century), also Arnobius the Elder, Roman rhetorician in the province of Africa

Domitius Afer (d. 60), Roman orator

Ptilostomus afer, piapiac

Nilaus afer, Brubru

AFER, an alternate acronym for the Air Force Expeditionary Service Ribbon

Cinnyris afer, Greater Double-collared Sunbird

Publius Aelius Hadrianus Afer, father of the Roman emperor Hadrian

Afer, an individual of the Afri tribe after which the continent of Africa is probably named

Francolinus afer, Red-necked Francolin

Euplectes afer, Yellow-crowned Bishop

Turtur afer, Blue-spotted Wood Dove

Parus afer, Southern Grey Tit, also classified as Melaniparus afer

Afer, a roman Cognomen in reference to Africa, see list of Roman cognomina.