Alzheimer's Family Organization - health support group in Florida

"Axial Force Only" - Engineering term

"Confined Animal Feeding Operation" (CAFO) - Factory farming term

Association of Field Ornithologists - ornithology organization, publishers of Journal of Field Ornithologists

Agencia Filatelica Oficial - Spanish: Official Philatelic Agency, Barcelona

"American Family Online" (afo.net) - filtered internet service provider founded by American Family Association

A.F.O. game for Commodore PET

Accounting and finance office / Accounting and finance officer - see also Chief financial officer (CFO)

AFO is a three-letter acronym that may refer to:

All for One

Academia Film Olomouc - annual documentary films festival in Olomouc, Czech Republic

Aero Force One - Aerosmith fan club

Air Force One - air traffic control call sign of any United States Air Force aircraft carrying the President of the United States

Air Force 1 (shoe) - Nike, Inc. shoe design

Air Force Ones (song) - 2002 song written and performed by St. Louis, Missouri hip hop artist Nelly

Alien Front Online - Sega video game

Anime Festival Orlando - yearly anime convention in Orlando, Florida

Anti-Fascist Organisation - provisional organization in Burma during World War II

Arellano-Felix Organization - drug trafficking cartel AKA Tijuana Cartel in Mexico

Armenian Sculpturer Rafik Khachatryan - Khachar

AFO (Canada) - L'assemblée de la Francophonie de l'Ontario, a cultural and political organization for Franco-Ontarians in the Canadian province of Ontario

Australian Field Ornithology - journal of Bird Observation & Conservation Australia

Authorised Firearms Officer - police officer in the United Kingdom who has received training and authorization to carry and use firearms