Berg was born in Brooklyn, New York, one of three sons of Harry Berg, a clothing manufacturer, and Sarah Brodsky, a homemaker. He attended public schools, including Abraham Lincoln High School, from which he graduated in 1943. In a 1980 interview reported in the New York Times, Berg credited a "Mrs. Wolf," the woman who ran a science club after school, with inspiring him to become a researcher. He graduated from high school with a keen interest in microbiology and entered Pennsylvania State University, where he received a degree in biochemistry in 1948.
Before entering graduate school, Berg served in the United States Navy from 1943 to 1946. On September 13, 1947, he married Mildred Levy; the couple later had one son. After completing his duty in the navy, Berg continued his study of biochemistry at Western Reserve University (now Case Western Reserve University) in Cleveland, Ohio, where he was a National Institutes of Health fellow from 1950 to 1952 and received his doctorate degree in 1952. He did postdoctoral training as an American Cancer Society research fellow, working with Herman Kalckar at the Institute of Cytophysiology in Copenhagen, Denmark, from 1952 to 1953. From 1953 to 1954 he worked with biochemist Arthur Kornberg at Washington University in St.
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