Oswald Theodore Avery Biography

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World of Chemistry on Oswald Theodore Avery

By the early 1940s, scientists knew that chromosomes existed and that they were composed of smaller units called genes. Chemical analysis had revealed that the eucaryotic chromosome consists of about 50% protein and 50% deoxyribonucleic acid ( DNA). There was no particular interest in DNA for several previous decades because no role had been assigned to it. This changed when a Canadian-born American named Oswald Avery showed that DNA is responsible for the transmission of heritable characteristics.

Avery moved from Canada to New York City in 1887. He attended Colgate University, and in 1904, he received his medical degree from the College of Physicians and Surgeons at Columbia University. He practiced medicine for several months before he became more interested in the transmission of infectious diseases. In 1913, Avery arrived at Rockefeller Institute, where he worked as a bacteriologist for over 43 years.

Avery and his coworkers studied the life cycle and chemical make-up of Diplococcus pneumoniae, or pneumonocci, a species of bacteria that causes pneumonia. Avery's interest was sparked by the work of Frederick Griffith, and by 1932 Avery focused on transformation--a process by which heritable characteristics of one species are incorporated into another different species. In 1928, Griffith described an experiment in which he injected mice with a mixture of a harmless strain of living pneumonococci and the dead remains of a virulent strain of the bacteria. The mice died from infection by the live organisms of the virulent strain, though they had been dead when they were administered.

In an attempt to duplicate Griffith's work, Avery and his colleagues began to grow large quantities of virulent type III capsulated pneumonococcus. They purified the live virulent encapsulated bacteria and then killed them by extreme heat. The bacteria's polysaccharide protein that makes up the capsule or outer envelope was then removed. The remaining portion of the dead bacteria, its polysaccharide gone but capsules intact, was added to living, unencapsulated bacteria. It was found that the offspring of these bacteria had capsules. Avery had determined that the active transforming principle, as Griffith had described earlier, still remained. Because the polysaccharide protein had been removed for the test, it could not be the transforming factor.

Avery wanted to be certain that the active agent was the DNA and not a small amount of protein contamination. To verify the result, a quantity of DNase, an enzyme that would destroy the DNA without affecting the protein, was prepared and added to the sample. When a portion of bacteria was tested, it could no longer transform the unencapsulated bacteria into encapsulated bacteria. Avery and his coworkers had conclusively proven that DNA was the transforming principle responsible for the development of polysaccharide capsules in the unencapsulated bacteria.

This experiment, first published in 1944, was extremely important because, for the first time, scientists had proven that DNA controls the development of a cellular feature. It also implicated DNA as the basic genetic material of cells and stimulated James Watson and Francis Crick to later discover its structure and method of replication. Today, we understand that DNA is the fundamental molecule involved in heredity.

Dr. Avery also worked in the field of immunology, where he determined that polysaccharides play an important role in immunity and helped to develop diagnostic tests used to identify many disease-causing bacteria. He received many awards and honors throughout his distinguished career, including induction into the prestigious National Academy of Sciences and the Royal Society of London.