Density gradient centrifugation is a technique that allows the separation of cells, organelles and macromolecules, depending on their size, shape and density. A density gradient is created in a centrifuge tube by layering solutions of varying densities...

Sucrose gradient centrifugation is a type of centrifugation often used to purify enveloped viruses (with densities 1.1-1.2 g/cm³) and ribosomes, and also to separate cell organelles from crude cellular...

The molecular motors myosin-V and kinesin form a complex to transport vesicles within the neuron. Vesicles move long distances along microtubule tracks using kinesin motors and then switch to actin filaments for short distance movement using myosin-V motors. Affinity isolation of native kinesin...

Evolution of Sucrose Synthesis[w ] Cyanobacteria and proteobacteria (purple bacteria) are the only prokaryotes known to synthesize sucrose (Suc). Suc-P synthase, Suc-phosphatase (SPP), and Suc synthase activities have previously been detected in several cyanobacteria, and genes coding for Suc-P synthase (sps) and Suc...