Primer can refer to:
- Primer (molecular biology), a nucleic acid strand (or related molecule) that serves as a starting point for DNA replication
- Percussion cap, in ballistics, a small charge that explodes when struck with force or heated with electricity, typically to ignite a larger charge of propellant
- Explosive primer
- Primer (paint), a coating applied to a surface to prepare it for paint or another coating or adhesive
- Primer (textbook) A textbook formerly used in primary education (often pronounced "primmer" in General American) to teach the alphabet and other basic concepts
- New England Primer, contained moral-themed rhymes associated with the colonial American Puritan culture
- An old English word for a Book of Hours
- Primer (film), a 2004 feature film written and directed by Shane Carruth
- Primer (gasoline engine), a device on some gasoline engines used to prime the engine with gasoline before starting it
- Primer (comic), a comic book published by Comico Comics
- Great primer, a typefeace about 18 points, or long primer, about 10 points
- Primer Artist Co-operative, founded in 2007
- Primer-E Primer, A software for statistical analysis of ecological data.
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