Molecular biologists use a specialized technique termed nick translation to label double stranded DNA molecules. The technique prepares the DNA molecules for further blotting analysis studies (e.g., Southern and Northern blotting analysis). The nick...

Nick translation is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in Fluorescent...