Gene cloning, or molecular cloning, has several different meanings to a molecular biologist. A clone is an exact copy, or replica, of something. In the literal sense, cloning a gene means to make many exact copies of a segment of a DNA molecule that...

[Molecular cloning] refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo. Cloning is frequently employed to amplify DNA fragments containing genes, but it can be used to amplify any DNA sequence such as...

ABSTRACT: Phospholipase A^sub 2^ (PLA^sub 2^) activity was investigated in various tissues of tobacco (Nicotiana tabacum). PLA^sub 2^ activity in the flower was 15 times higher than that in the leaf, stem, and root. PLA^sub 2^ activity in the flower appears to have originated...

Soybean [Glycine max (L.) Merr.] is a good protein source for both humans and livestock. However, soybean seed proteins are deficient in the sulfur-containing amino acids cysteine and methionine. This deficiency has stimulated efforts to improve the amino acid composition of soybean seed proteins....