Heteroduplex analysis (HA) is a method of detecting sequence differences between a reference DNA and a test DNA (both usually produced by polymerase chain reaction amplification). It is commonly used to detect mutations in a gene, and is much simpler...

A heteroduplex is a double-stranded (duplex) molecule of nucleic acid originated through the genetic recombination of single complementary strands derived from different sources, such as from different homologous chromosomes or even from different...

General DNA analysis includes detection of targets, as well as identification and quantification of specific variants. Genomic DNA exists in the form of hotnoduplexes, with all corresponding base pairs being complementary, A:T and C:G. We call a double-stranded DNA (dsDNA) molecule a heteroduplex when...

ABSTRACT Meiotic recombination gives rise to crossovers, which are required in most organisms for the faithful segregation of homologous chromosomes during meiotic cell division. Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover formation. We report...